Molecular Analysis of the A.eutrophus (PHB) Biosynthetic Operon:Identification of the N Terminus of PHB Synthase &Identification of the Promoter

- P(3HB) = PHB
- This paper is to analyze phbC (by using molecular technique method)
- Analysis:

• Identify translational initiation codon (from cell R.eutrophathat has phbC'-'lac fusion gene)
• the methods: analysis of gel electrophoretic homogeneity by chromatography on DEAE-Sephacel and on aminophenyl-B-D-thiogalacto-pyranosidase-sepharose

- The method of synthesis (Enzymatic reactions):

1. 2 acetyl-CoA (starter compound) --> (condensation) acetoacetyl-CoA
2. acetoacetyl-CoA --> (chiral reduction) D-(-)-3-hydroxy-butyryl-CoA
3. polymerization of D-(-)-3-hydroxybutyrate units